• Design of CRISPR editing platforms (CRIPR/Cas, base editing, prime editing, epigenome editing)
  • Cloning of expression cassettes, donor vectors, delivery vectors, synthetic genes
  • Design and production of viral and non viral delivery tools (lentiviral vectors, Adeno-Associated Viral Vectors, Virus-Like Particles)
  • Protein and receptor engineering
  • RNA and Protein quantification (qPCR, ddPCR, immunooblotting)
  • In vitro 3D organoid models of the blood brain barrier
  • Isolation of hematopoietic stem cells, Immune cells, CNS and PNS cell types from human and murine samples
  • Primary cell culture systems
  • Colony-Forming Unit assays, proliferation and migration assays
  • Mouse handling, breeding and related procedures
  • CRISPR safety and toxicology assessment (off targets sequencing, bystander editing assessment, in vivo biodistribution, in vivo immunology assessment)
  • Imaging of complex tissues (immunohistochemistry, confocal analysis)
  • Immunophenotyping (flow cytometry, cell sorting, magnetic beads sorting)
  • Omics analyses (RNA-seq, scRNA-seq, ChIP-seq, ATAC-seq, spatial transcriptomics)